Pointilistic Portraits Whereas STED and SIM...

16
May

$Pointilistic Portraits Whereas STED and SIM regulate the location of fluorophore emissions, PALM and STORM use time to separate individual particles at super-resolution. PALM and STORM apply the excitation light at a sufficiently low intensity so that only one molecule in the diffraction-limited region emits light. This process is repeated thousands of times, and the localized “points” are assembled together into a high-resolution image, in a similar manner as the “pointillism” painting technique.$

Pointilistic Portraits

Whereas STED and SIM regulate the location of fluorophore emissions, PALM and STORM use time to separate individual particles at super-resolution. PALM and STORM apply the excitation light at a sufficiently low intensity so that only one molecule in the diffraction-limited region emits light. This process is repeated thousands of times, and the localized “points” are assembled together into a high-resolution image, in a similar manner as the “pointillism” painting technique.

cell.com Short URL

#science #image #portrait #sted #sim #palm #storm #pointilism