16
May
Pointilistic Portraits

Whereas STED and SIM regulate the location of fluorophore emissions, PALM and STORM use time to separate individual particles at super-resolution. PALM and STORM apply the excitation light at a sufficiently low intensity so that only one molecule in the diffraction-limited region emits light. This process is repeated thousands of times, and the localized “points” are assembled together into a high-resolution image, in a similar manner as the “pointillism” painting technique.

Pointilistic Portraits

Whereas STED and SIM regulate the location of fluorophore emissions, PALM and STORM use time to separate individual particles at super-resolution. PALM and STORM apply the excitation light at a sufficiently low intensity so that only one molecule in the diffraction-limited region emits light. This process is repeated thousands of times, and the localized “points” are assembled together into a high-resolution image, in a similar manner as the “pointillism” painting technique.

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