16
May
Pointilistic Portraits Whereas STED and SIM regulate the location of fluorophore emissions, PALM and STORM use time to separate individual particles at super-resolution. PALM and STORM apply the excitation light at a sufficiently low intensity so that only one molecule in the diffraction-limited region emits light. This process is repeated thousands of times, and the localized “points” are assembled together into a high-resolution image, in a similar manner as the “pointillism” painting technique.

Pointilistic Portraits

Whereas STED and SIM regulate the location of fluorophore emissions, PALM and STORM use time to separate individual particles at super-resolution. PALM and STORM apply the excitation light at a sufficiently low intensity so that only one molecule in the diffraction-limited region emits light. This process is repeated thousands of times, and the localized “points” are assembled together into a high-resolution image, in a similar manner as the “pointillism” painting technique.

cell.com Permalink 15 Notes Short URL
#science #image #portrait #sted #sim #palm #storm #pointilism

About This Blog

SCIENCE has explained nothing; the more we know the more fantastic the world becomes and the profounder the surrounding darkness.

Aldous Huxley, 1894-1963.

This blog resides firmly at the intersection of scientific research, education, art, and communication. Herein lies information and current happenings related to each, as well as any other sciencey goodness worth sharing.

About Me

Hi there, I'm Jim: PhD student in the biological sciences, enthusiast, friendly neighbour, Australian.

Postcards from the lab

Contact Me

rationaldiscoveryblog@gmail.com

Follow me on Twitter